Repair Kinetics of DNA-DSB Induced by X-rays or Carbon Ions under Oxic and Hypoxic Conditions | Zendy

Ryoichi Hirayama | Zendy; Yoshiya Furusawa | Zendy; Takeshi Fukawa | Zendy; Koichi Andō | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Repair Kinetics of DNA-DSB Induced by X-rays or Carbon Ions under Oxic and Hypoxic Conditions

Author(s) -

Ryoichi Hirayama,

Yoshiya Furusawa,

Takeshi Fukawa,

Koichi Andō

Publication year - 2005

Publication title -

journal of radiation research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.643

H-Index - 60

eISSN - 1349-9157

pISSN - 0449-3060

DOI - 10.1269/jrr.46.325

Subject(s) - irradiation , dna , kinetics , ion , oxygen enhancement ratio , linear energy transfer , oxygen , biophysics , dna damage , carbon fibers , incubation , chemistry , radiochemistry , microbiology and biotechnology , materials science , biology , biochemistry , physics , organic chemistry , quantum mechanics , composite number , nuclear physics , composite material

We studied the relation between initial DNA double-strand breaks (DNA-DSB) and the rejoining kinetics of the strand breaks, as well as the OER (oxygen enhancement ratio) after low- and high-LET (linear energy transfer) radiations. CHO cells were exposed to 200 kVp X-rays or 80 keV/microm carbon ions under oxic and hypoxic conditions. DNA-DSB in the cells were analyzed by a static-field gel electrophoresis (SFGE). The kinetics of the rejoining could be described by a sum of fast and slow components. The initial released DNA after X-ray irradiation was higher for cells irradiated under an oxic condition than that under a hypoxic condition. The OER of DNA-DSB after X-ray irradiation was 5.7. This value decreased rapidly to be 3.4 with the fast component by 15 minutes. On the other hand, the OER of DNA-DSB after carbon ion irradiation was 2.2, and this value was not changed by rejoining incubation. The OER values for cell killing were 2.8 and 1.8 after X-ray and carbon ion irradiations, respectively. These values matched to the OER for DNA-DSB with complete rejoining. We conclude that the rejoining of DNA-DSB is an important factor in the mechanism of the oxygen effect.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research