Cellular Response to Pulsed Low-dose Rate Irradiation in X-ray Sensitive Hamster Mutant Cell Lines | Zendy

Natasja Castro Kreder | Zendy; Rosemarie ten Cate | Zendy; Hans M. Rodermond | Zendy; Chris van Bree | Zendy; Nicolaas A.P. Franken | Zendy; M.Z. Zdzienicka | Zendy; J. Haveman | Zendy

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Cellular Response to Pulsed Low-dose Rate Irradiation in X-ray Sensitive Hamster Mutant Cell Lines

Author(s) -

Natasja Castro Kreder,

Rosemarie ten Cate,

Hans M. Rodermond,

Chris van Bree,

Nicolaas A.P. Franken,

M.Z. Zdzienicka,

J. Haveman

Publication year - 2004

Publication title -

journal of radiation research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.643

H-Index - 60

eISSN - 1349-9157

pISSN - 0449-3060

DOI - 10.1269/jrr.45.385

Subject(s) - mutant , dna repair , homologous recombination , dna damage , irradiation , microbiology and biotechnology , chinese hamster , non homologous end joining , xrcc3 , cell culture , clonogenic assay , dna , biology , chemistry , genetics , gene , genotype , physics , nuclear physics , single nucleotide polymorphism

The role of DNA repair mechanisms in the cellular response to low dose rate (LDR) irradiation was studied with the aim to gain insight in the process of sublethal damage (SLD) repair. Chinese hamster cell lines mutated in either DNA single strand break (ssb) repair or DNA double strand break (dsb) repair by non homologous end joining (NHEJ) and homologous recombination (HR), or showing an AT-like phenotype, were irradiated in plateau-phase either at high dose rate (HDR, 3.3 Gy/min) or at pulsed low dose rate (p-LDR, average 1 Gy/h). Cell survival after irradiation was assessed using the clonogenic assay. A change in sensitivity when the dose rate was decreased was observed for all parental cell lines and the DNA ssb repair mutant. No difference in cell survival after p-LDR versus. HDR irradiation was observed for the two NHEJ mutants, the AT-like mutant and the HR mutant. Based on these results we conclude that single strand break repair does not play a role in the dose rate effect. The AT like protein, functional NHEJ and XRCC3 are required for the dose rate effect.

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