Detection of γ-Irradiation Induced DNA Damage and Radioprotection of Compounds in Yeast Using Comet Assay | Zendy

Purva Nemavarkar | Zendy; Bishnavath K. Chourasia | Zendy; K. Pasupathy | Zendy

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Detection of γ-Irradiation Induced DNA Damage and Radioprotection of Compounds in Yeast Using Comet Assay

Author(s) -

Purva Nemavarkar,

Bishnavath K. Chourasia,

K. Pasupathy

Publication year - 2004

Publication title -

journal of radiation research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.643

H-Index - 60

eISSN - 1349-9157

pISSN - 0449-3060

DOI - 10.1269/jrr.45.169

Subject(s) - comet assay , dna damage , yeast , irradiation , chemistry , comet , dna , biology , biochemistry , astrobiology , physics , nuclear physics

The single cell gel electrophoresis assay (SCGE), a very rapid and sensitive method, has been applied to follow gamma-irradiation induced DNA damage in budding yeast, Saccharomyces cerevisiae. Spheroplasting the gamma-irradiated yeast cells by enzyme glusulase, before subjecting them to electrophoresis, resulted in a well-defined appearance of comets. Yeast comets look quite different from mammalian comets. A linear relationship was observed between the doses of irradiation and the tail moments of comets. These studies were extended to follow the action of known radio-protectors, i.e., caffeine and disulfiram. The results revealed the usefulness SCGE as applied to yeast in studies of the gamma-irradiation-induced DNA breaks and also radio-protection by chemicals at doses that are not feasible with other eukaryotes.

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