Detection of DNA strand breaks in barley roots after .GAMMA.-ray irradiation using the alkaline unwinding assay. | Zendy

Naoya Shikazono | Zendy; Hiroshi Watanabe | Zendy; Atsushi Tanaka | Zendy; Shigemitsu Tano | Zendy; Atsushi Hirai | Zendy

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Detection of DNA strand breaks in barley roots after .GAMMA.-ray irradiation using the alkaline unwinding assay.

Author(s) -

Naoya Shikazono,

Hiroshi Watanabe,

Atsushi Tanaka,

Shigemitsu Tano,

Atsushi Hirai

Publication year - 1994

Publication title -

journal of radiation research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.643

H-Index - 60

eISSN - 1349-9157

pISSN - 0449-3060

DOI - 10.1269/jrr.35.35

Subject(s) - irradiation , dna , single strand , incubation , chemistry , double strand , gamma irradiation , biophysics , radiochemistry , exogenous dna , microbiology and biotechnology , biochemistry , dna damage , biology , physics , nuclear physics

The alkaline unwinding assay was applied to measure strand breaks in DNA of intact barley roots after gamma-ray irradiation without extracting DNA from the tissue. The most suitable condition for unwinding was determined to be 0.03 M NaOH containing 0.5 M NaCl for alkaline solution composition, and 15 min at 20 degrees C for unwinding time. Under this condition, DNA strand breaks were shown to be linearly increased with increasing dose, and further be rejoined during the post-irradiation incubation for 6 hr.

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