Significance of the Proportion of Binucleate Cells in the Micronucleus Assay: A Methodological Study. | Zendy

Masahiro Imamura | Zendy; Margareta Edgren | Zendy

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Significance of the Proportion of Binucleate Cells in the Micronucleus Assay: A Methodological Study.

Author(s) -

Masahiro Imamura,

Margareta Edgren

Publication year - 1994

Publication title -

journal of radiation research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.643

H-Index - 60

eISSN - 1349-9157

pISSN - 0449-3060

DOI - 10.1269/jrr.35.11

Subject(s) - micronucleus test , micronucleus , cytochalasin b , glutathione , buthionine sulfoximine , microbiology and biotechnology , chinese hamster , biology , andrology , chemistry , cell culture , toxicology , biochemistry , cell , genetics , medicine , toxicity , enzyme

Using treatment with cytochalasin-B (Cyt-B) for the induction of a cytokinetic block, the significance of the proportion of binucleate cells (BNC) in the micronucleus (MN) assay was investigated in a methodological study. A Chinese hamster cell line V79 was used in which MN were induced by radiation. In complementary tests the radiation effect in inducing MN was enhanced by depletion of the cellular glutathione content with buthionine sulfoximine (BSO). The data indicated that the concentration of Cyt-B is the major factor which determines the proportion of BNC. This proportion was shown to be independent of radiation dose and of BSO. Furthermore, the MN frequency was not related to the percentage of BNC. Therefore, a high proportion of BNC may be practical for the MN assay, but may not make the technique more accurate.

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