DNA repair characteristics of a hybrid cell clone between xeroderma pigmentosum and Potorous tridactilis. | Zendy

Kenji Ida | Zendy

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DNA repair characteristics of a hybrid cell clone between xeroderma pigmentosum and Potorous tridactilis.

Author(s) -

Kenji Ida

Publication year - 1986

Publication title -

journal of radiation research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.643

H-Index - 60

eISSN - 1349-9157

pISSN - 0449-3060

DOI - 10.1269/jrr.27.300

Subject(s) - xeroderma pigmentosum , microbiology and biotechnology , cell culture , biology , chromosome , dna , chemistry , gene , genetics , dna repair

A hybrid cell clone PX1 was isolated by fusing UV sensitive XP20S(SV)neo, an SV-40-transformed, neomycin-resistant xeroderma pigmentosum (XP) cell line, and Pt K2, a rat kangaroo (Potorous tridactilis) cell line. The UV-survival curve of PX1 cells fell midway between those of Pt K2 and XP20S(SV)neo cells, since mean lethal doses(D0) were 2.5, 4.7 and 0.27 J/m2 for PX1, Pt K2 and XP20S(SV)neo, respectively. Amounts of unscheduled DNA synthesis (UDS) after UV, relative to normal human cells, were 60.4% for Pt K2, 37.7% for PX1 and 0.1% for XP20S(SV)neo. Such relative UDS capacities for excision repair of Pt K2, PX1 and XP20S(SV)neo were also consistent with the respective relative capacities of host cell reactivation (HCR) of UV-irradiated Herpes simplex virus. Apparently, there was no single Pt K2 chromosome in the PX1 cells. One possibility is that a gene which may account for the partial restoration of the UV resistance has been transferred from Pt K2 to PX1.

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