Block of cleavage of ultraviolet-irradiated DNA with restriction endonucleases. | Zendy

Kang Wei | Zendy; Satoru Tomita | Zendy; Kenshi Suzuki | Zendy

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Block of cleavage of ultraviolet-irradiated DNA with restriction endonucleases.

Author(s) -

Kang Wei,

Satoru Tomita,

Kenshi Suzuki

Publication year - 1986

Publication title -

journal of radiation research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.643

H-Index - 60

eISSN - 1349-9157

pISSN - 0449-3060

DOI - 10.1269/jrr.27.247

Subject(s) - cleavage (geology) , restriction enzyme , pbr322 , hindiii , dna , chemistry , pyrimidine dimer , ultraviolet light , ultraviolet , dimer , plasmid , stereochemistry , microbiology and biotechnology , biology , biochemistry , photochemistry , dna damage , physics , optics , paleontology , organic chemistry , fracture (geology)

Plasmid pBR322 DNA was irradiated with ultraviolet light at 254 nm and digested with various restriction endonucleases. Analyses of the products suggested that the pyrimidine dimers which would have been formed just across the cutting site (Acc I), at the opposite position of the cutting site (Hind III, Eco RI, Bam HI), and at the position adjacent to the cutting site (Aha III) blocked the cleavage with the enzymes. In addition, experiments with Hind III, Eco RI and Bam HI showed that the extent of cleavage block appears to reflect the relative efficiency of dimer formation in sequences, TT, CT and CC.

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