Effects of cell concentrations on the survival and repopulation of haemopoietic stem cells in irradiated bone marrow cell culture in vitro. | Zendy

H Fujitake | Zendy; Yukari Okamoto | Zendy; H Okubo | Zendy; Takeshi Miyanomae | Zendy; Kazuaki Kumagai | Zendy; Kazuhiro Mori | Zendy

Open Access

Effects of cell concentrations on the survival and repopulation of haemopoietic stem cells in irradiated bone marrow cell culture in vitro.

Author(s) -

H Fujitake,

Yukari Okamoto,

H Okubo,

Takeshi Miyanomae,

Kazuaki Kumagai,

Kazuhiro Mori

Publication year - 1981

Publication title -

journal of radiation research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.643

H-Index - 60

eISSN - 1349-9157

pISSN - 0449-3060

DOI - 10.1269/jrr.22.449

Subject(s) - stem cell , bone marrow , repopulation , stromal cell , in vitro , biology , cell culture , cell , bone marrow stem cell , stem cell factor , andrology , cell growth , immunology , haematopoiesis , microbiology and biotechnology , cancer research , medicine , biochemistry , genetics

Effects of cell concentrations on the survival and repopulation of haemopoietic stem cells after irradiation were studied in the long-term culture of mouse bone marrow cells in vitro. No difference was observed in the survival of the stem cells among cultures in which 0-107 cells were re-inoculated on the adherent cell colonies in the culture flask. Stem cells showed a significant proliferation within 1 week and the number of the stem cells exceeded the control in 3 weeks after irradiation in the cultures with less than 106 re-inoculated cells per flask. In contrast, there was a considerable delay in the onset of stem cell proliferation after irradiation in the culture with 107 cells per flask. Based on these results, a possiblity that a stimulator of stem cell proliferation, released from irradiated stromal cells, is cancelled by an inhibitory factor produced by irradiated or unirradiated haemopoietic cells is postulated.

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