Lack of Recovery from Radiation Damage on Colony Forming Ability and on Membrane Charge in a Burkitt Lymphoma Cell Line

*佐 藤 周子:愛 知県 がんセ ンター研究所放射線部 ,名 古屋市千種区田代町 〒464 ** 小 島清秀:愛知県がんセンター研究所 生物部 松 沢 大樹:東 北大学抗酸菌病研究所 ,放 射線部,仙 台市広瀬 町4-12 〒980 西 連寺 剛,日沼頼夫:熊 本大学医学部微生物学教室 ,熊 本市本荘2-2-1〒860 A Burkitt lymphoma cell line, P3HR-1, was found to be very radiaosensitive (n=1, D0=67R) and showed no substantial recovery by dose-fractionation. Measuremant of electrophoretic mobility of whole cells and isolated nuclei after irradiation demonstrated that the loss of negative charge on the membrane was irreversible in this cell line. Analysis of frequency distribution pattern of electrophoretic mobility indicated a good correlation between the loss of colony forming ability and the irreversible loss of membrane charge. The surface saccharides responsible for electrokinetic behavior of the P3HR-1 cells mainly consisted of sialic acid and chondroitine sulfate, but not of hyaluronic acid. The mobility reduction by the removal of these saccharides was reversible and did not result in cell death.