Crucial Role of SDF-1/CXCR4 Interaction in the Recruitment of Transplanted Dermal Multipotent Cells to Sublethally Irradiated Bone Marrow | Zendy

Zhaowen Zong | Zendy; Tianmin Cheng | Zendy; Yongping Su | Zendy; Xinze Ran | Zendy; Nan Li | Zendy; Guoping Ai | Zendy; Hui Xu | Zendy

Open Access

Crucial Role of SDF-1/CXCR4 Interaction in the Recruitment of Transplanted Dermal Multipotent Cells to Sublethally Irradiated Bone Marrow

Author(s) -

Zhaowen Zong,

Tianmin Cheng,

Yongping Su,

Xinze Ran,

Nan Li,

Guoping Ai,

Hui Xu

Publication year - 2006

Publication title -

journal of radiation research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.643

H-Index - 60

eISSN - 1349-9157

pISSN - 0449-3060

DOI - 10.1269/jrr.0531

Subject(s) - bone marrow , stromal cell , haematopoiesis , cxcr4 , andrology , transplantation , chemotaxis , endogeny , in vitro , incubation , stem cell , chemistry , receptor , immunology , biology , microbiology and biotechnology , cancer research , medicine , inflammation , endocrinology , chemokine , biochemistry

Our previous study indicated that dermal multipotent cells (DMCs) could engraft into bone morrow (BM) of rats with sublethal irradiation and promote hematopoietic recovery after being transplanted systemically, but the mechanisms determining the recruitment of DMCs to the irradiation injured BM remain unclear. In the present study, we investigated the role of stromal cellderived factor-1 (SDF-1)/CXCR4 interaction in this process. Male DMCs were isolated and transplanted into female rats systemically, and by employing quantitative real-time TaqMan polymerase chain reaction for the sex-determining region of Y chromosome, it was found that the amount of DMCs in BM of rats with sublethal irradiation was about 3 times more than that of normal rats (P < 0.01). Incubation of DMCs with AMD3100 before transplantation, which specifically blocks binding of SDF-1 to its endogenous receptor CXCR4, diminished recruitment of DMCs to the injured BM by 57.2 +/- 5.5% (P < 0.05). In addition, it was confirmed that the expression of SDF-1 in injured BM was up-regulated when compared with that in normal BM, and in vitro analysis revealed that BM extracts from irradiated rats had a strong chemotactic effect on DMCs, which decreased significantly when DMCs were pre-incubated with AMD3100 (P < 0.05). These data suggest that transplanted DMCs were recruited more frequently to irradiation-injured BM than normal BM and the interactions of SDF-1/CXCR4 played an important role in this process.

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