Open AccessSARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay
Author(s) -
Michael D. Buck,
Enzo Z. Poirier,
Ana Cardoso,
Bruno Frederico,
Johnathan Canton,
Sam Barrell,
Rupert Beale,
Richard Byrne,
Simon Caidan,
Margaret Crawford,
Laura Cubitt,
Sonia Gandhi,
Robert Goldstone,
Paul Grant,
Kiran Gulati,
Steve Hindmarsh,
Michael Howell,
Michael Hubank,
Rachael Instrell,
Ming Jiang,
George Kassiotis,
Wei-Ting Lu,
James I. MacRae,
Iana Martini,
Davin P. Miller,
David Moore,
Eleni Nastouli,
Jérôme Nicod,
Luke Nightingale,
Jessica Olsen,
Amin Oomatia,
Nicola O’Reilly,
Anett Rideg,
OkRyul Song,
Amy Strange,
Charles Swanton,
Samra Turajlic,
Mary Wu,
Caetano Reis e Sousa
Publication year - 2021
Publication title -
wellcome open research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.298
H-Index - 21
ISSN - 2398-502X
DOI - 10.12688/wellcomeopenres.16517.2
Subject(s) - covid-19 , medicine , pandemic , diagnostic test , virology , pathology , emergency medicine , disease , infectious disease (medical specialty)
The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority of patient samples is assessed for viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals and an accredited clinical diagnostic laboratory, established a diagnostic testing pipeline that has reported on more than 252,000 RT-qPCR results since its commencement at the beginning of April 2020. However, due to ongoing demand and competition for critical resources, alternative testing strategies were sought. In this work, we present a clinically-validated procedure for high-throughput SARS-CoV-2 detection by RT-LAMP that is robust, reliable, repeatable, specific, and inexpensive.