Scaled deployment of Wolbachia to protect the community from dengue and other Aedes transmitted arboviruses

: A number of new technologies are under development for the control of mosquito transmitted viruses, such as dengue, chikungunya and Zika that all require the release of modified mosquitoes into the environment. None of these technologies has been able to demonstrate evidence that they can be implemented at a scale beyond small pilots. Here we report the first successful citywide scaled deployment of in the northern Australian city of Townsville. : The Mel strain of was backcrossed into a local genotype and mass reared mosquitoes were deployed as eggs using mosquito release containers (MRCs). In initial stages these releases were undertaken by program staff but in later stages this was replaced by direct community release including the development of a school program that saw children undertake releases. Mosquito monitoring was undertaken with Biogents Sentinel (BGS) traps and individual mosquitoes were screened for the presence of with a Taqman qPCR or LAMP diagnostic assay. Dengue case notifications from Queensland Health Communicable Disease Branch were used to track dengue cases in the city before and after release. : was successfully established into local mosquitoes across 66 km in four stages over 28 months with full community support.  A feature of the program was the development of a scaled approach to community engagement. frequencies have remained stable since deployment and to date no local dengue transmission has been confirmed in any area of Townsville after has established, despite local transmission events every year for the prior 13 years and an epidemiological context of increasing imported cases. : Deployment of into populations can be readily scaled to areas of ~60km quickly and cost effectively and appears in this context to be effective at stopping local dengue transmission.