A novel multiplex PCR for detection of Pseudomonas aeruginosa: A major cause of wound infections
Author(s) -
Muhammad Salman,
Aamir Ali,
Abdul Haque
Publication year - 2013
Publication title -
pakistan journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.316
H-Index - 30
eISSN - 1682-024X
pISSN - 1681-715X
DOI - 10.12669/pjms.294.3652
Subject(s) - pseudomonas aeruginosa , multiplex polymerase chain reaction , microbiology and biotechnology , multiplex , medicine , pseudomonas exotoxin , gene , polymerase chain reaction , bacteria , biology , bioinformatics , genetics
Wound infections are often difficult to treat due to various bacterial pathogens. Pseudomonas aeruginosa is one of the common invaders of open wounds. Precise diagnosis of this etiological agent in wound infections is of critical importance particularly in treatment of problematic cases. The existing diagnostic methods have certain limitations particularly related to specificity. Our objective was to to establish a comprehensive and reliable multiplex PCR to confirm diagnosis of P. aeruginosa.
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