Small molecule control of pre-mRNA splicing | Zendy

Brenton R. Graveley | Zendy

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Small molecule control of pre-mRNA splicing

Author(s) -

Brenton R. Graveley

Publication year - 2005

Publication title -

rna

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.037

H-Index - 171

eISSN - 1469-9001

pISSN - 1355-8382

DOI - 10.1261/rna.7229705

Subject(s) - rna splicing , sr protein , biology , spliceosome , exonic splicing enhancer , alternative splicing , protein splicing , polypyrimidine tract , microbiology and biotechnology , messenger rna , splicing factor , minigene , rna binding protein , precursor mrna , genetics , gene , rna

SR proteins regulate alternative splicing by binding to exonic sequences where, via an arginine/serine-rich splicing activation domain, they enhance the binding of the spliceosome to the adjacent splice sites. Here, a system is described in which a nontoxic derivative of the small molecule rapamycin is used to control pre-mRNA splicing in vitro. This involves the rapamycin-dependent recruitment of a splicing activation domain located on one protein to a second protein bound to the pre-mRNA. These results provide a new approach to explore for regulating gene expression in vivo with small molecules by controlling pre-mRNA splicing.

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