A novel chromatography system to isolate active ribosomes from pathogenic bacteria | Zendy

Bruce A. Maguire | Zendy; L Wondrack | Zendy; Leonard G. Contillo | Zendy; Zuoyu Xu | Zendy

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A novel chromatography system to isolate active ribosomes from pathogenic bacteria

Author(s) -

Bruce A. Maguire,

L Wondrack,

Leonard G. Contillo,

Zuoyu Xu

Publication year - 2007

Publication title -

rna

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.037

H-Index - 171

eISSN - 1469-9001

pISSN - 1355-8382

DOI - 10.1261/rna.692408

Subject(s) - ribosome , deinococcus radiodurans , biology , escherichia coli , bacteria , bacillus subtilis , microbiology and biotechnology , rna , biochemistry , genetics , gene

We have developed a novel chromatography for the rapid isolation of active ribosomes from bacteria without the use of harsh conditions or lengthy procedures that damage ribosomes. Ribosomes interact with an alkyl linker attached to the resin, apparently through their RNA component. Examples are given with ribosomes from Escherichia coli, Deinococcus radiodurans, and with clinical isolates of Streptococcus pneumoniae and methicillin-resistant Staphylococcus aureus (MRSA). The ribosomes obtained by this method are unusually intact, so that highly active ribosomes can now be isolated from the clinical isolates, enabling significantly improved in vitro functional assays that will greatly assist the discovery and development of new ribosomally targeted antibiotics.

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