A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells | Zendy

Peter T. Nelson | Zendy; Mariangels De Planell-Saguer | Zendy; Stella Lamprinaki | Zendy; Marianthi Kiriakidou | Zendy; Paul J. Zhang | Zendy; Una O’Doherty | Zendy; Zissimos P. Mourelatos | Zendy

Open Access

A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells

Author(s) -

Peter T. Nelson,

Mariangels De Planell-Saguer,

Stella Lamprinaki,

Marianthi Kiriakidou,

Paul J. Zhang,

Una O’Doherty,

Zissimos P. Mourelatos

Publication year - 2007

Publication title -

rna

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.037

H-Index - 171

eISSN - 1469-9001

pISSN - 1355-8382

DOI - 10.1261/rna.646007

Subject(s) - argonaute , biology , microrna , small interfering rna , monoclonal antibody , effector , microbiology and biotechnology , gene silencing , antibody , rna , gene , genetics

Argonaute (Ago) proteins bind to microRNA (miRNAs) and short interfering RNAs (siRNAs) and form the core components of effector complexes that mediate miRNA and siRNA function. Currently, there is a paucity of reliable antibodies against mammalian Ago proteins, thus precluding studies of endogenous Ago proteins from tissues. Here we report the development of 2A8, a novel anti-Ago monoclonal antibody that recognizes human and mouse Ago proteins and efficiently immunoprecipitates miRNAs. We report the characterization of 2A8 and its use to clone miRNAs from human brain and from preparations of human polymorphonuclear leukocytes (neutrophils), which revealed a prevalent miRNA with unusual features.

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