Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec | Zendy

Mahadev Rao | Zendy; Bradley A. Carlson | Zendy; Sergey V. Novoselov | Zendy; Donald P. Weeks | Zendy; Vadim N. Gladyshev | Zendy; Dolph L. Hatfield | Zendy

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Chlamydomonas reinhardtii selenocysteine tRNA^[Ser]Sec

Author(s) -

Mahadev Rao,

Bradley A. Carlson,

Sergey V. Novoselov,

Donald P. Weeks,

Vadim N. Gladyshev,

Dolph L. Hatfield

Publication year - 2003

Publication title -

rna

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.037

H-Index - 171

eISSN - 1469-9001

pISSN - 1355-8382

DOI - 10.1261/rna.5510503

Subject(s) - chlamydomonas reinhardtii , biology , transfer rna , selenocysteine , genetics , biochemistry , chlamydomonas , translation (biology) , gene , rna , enzyme , mutant , messenger rna , cysteine

Eukaryotic selenocysteine (Sec) protein insertion machinery was thought to be restricted to animals, but the occurrence of both Sec-containing proteins and the Sec insertion system was recently found in Chlamydomonas reinhardtii, a member of the plant kingdom. Herein, we used RT-PCR to determine the sequence of C. reinhardtii Sec tRNA[Ser]Sec, the first non-animal eukaryotic Sec tRNA[Ser]Sec sequence. Like its animal counterpart, it is 90 nucleotides in length, is aminoacylated with serine by seryl-tRNA synthetase, and decodes specifically UGA. Evolutionary analyses of known Sec tRNAs identify the C. reinhardtii form as the most diverged eukaryotic Sec tRNA[Ser]Sec and reveal a common origin for this tRNA in bacteria, archaea, and eukaryotes.

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