Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation | Zendy

Edward Horsey | Zendy; Jelena Jakovljevic | Zendy; Tiffany D. Miles | Zendy; Piyanun Harnpicharnchai | Zendy; John L. Woolford | Zendy

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Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation

Author(s) -

Edward Horsey,

Jelena Jakovljevic,

Tiffany D. Miles,

Piyanun Harnpicharnchai,

John L. Woolford

Publication year - 2004

Publication title -

rna

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.037

H-Index - 171

eISSN - 1469-9001

pISSN - 1355-8382

DOI - 10.1261/rna.5255804

Subject(s) - ribosomal protein , biology , ribosomal rna , ribosome biogenesis , eukaryotic large ribosomal subunit , 5s ribosomal rna , ribosome , 5.8s ribosomal rna , eukaryotic ribosome , 18s ribosomal rna , eukaryotic small ribosomal subunit , 50s , 23s ribosomal rna , 28s ribosomal rna , 30s , genetics , rna , microbiology and biotechnology , gene

The Saccharomyces cerevisiae gene RRP1 encodes an essential, evolutionarily conserved protein necessary for biogenesis of 60S ribosomal subunits. Processing of 27S pre-ribosomal RNA to mature 25S rRNA is blocked and 60S subunits are deficient in the temperature-sensitive rrp1-1 mutant. We have used recent advances in proteomic analysis to examine in more detail the function of Rrp1p in ribosome biogenesis. We show that Rrp1p is a nucleolar protein associated with several distinct 66S pre-ribosomal particles. These pre-ribosomes contain ribosomal proteins plus at least 28 nonribosomal proteins necessary for production of 60S ribosomal subunits. Inactivation of Rrp1p inhibits processing of 27SA(3) to 27SB(S) pre-rRNA and of 27SB pre-rRNA to 7S plus 25.5S pre-rRNA. Thus, in the rrp1-1 mutant, 66S pre-ribosomal particles accumulate that contain 27SA(3) and 27SB(L) pre-ribosomal RNAs.

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