Definition of bases in 23S rRNA essential for ribosomal subunit association | Zendy

Ülo Maiväli | Zendy; Jaanus Rèmme | Zendy

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Definition of bases in 23S rRNA essential for ribosomal subunit association

Author(s) -

Ülo Maiväli,

Jaanus Rèmme

Publication year - 2004

Publication title -

rna

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.037

H-Index - 171

eISSN - 1469-9001

pISSN - 1355-8382

DOI - 10.1261/rna.5220504

Subject(s) - ribosome , biology , 23s ribosomal rna , ribosomal rna , 50s , protein subunit , 30s , ribosomal protein , peptidyl transferase , eukaryotic ribosome , computational biology , translation (biology) , 5.8s ribosomal rna , genetics , microbiology and biotechnology , rna , messenger rna , gene

The ribosome is a two-subunit molecular machine, sporting a working cycle that involves coordinated movements of the subunits. Recent structural studies of the 70S ribosome describe a rather large number of intersubunit contacts, some of which are dynamic during translocation. We set out to determine which intersubunit contacts are functionally indispensable for the association of ribosome subunits by using a modification interference approach. Modification of the N-1 position of A715, A1912, or A1918 in Escherichia coli 50S subunits is strongly detrimental to 70S ribosome formation. This result points to 23S rRNA helices 34 and 69, and thus bridges B2a and B4, as essential for ensuring stability of the 70S ribosome.

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