A case for “StopGo”: Reprogramming translation to augment codon meaning of GGN by promoting unconventional termination (Stop) after addition of glycine and then allowing continued translation (Go) | Zendy

John F. Atkins | Zendy; Norma M. Wills | Zendy; Gary Loughran | Zendy; Chih-yu Wu | Zendy; Krishna Parsawar | Zendy; Martin D. Ryan | Zendy; Chung-Hsiung Wang | Zendy; Chad Nelson | Zendy

Open Access

A case for “StopGo”: Reprogramming translation to augment codon meaning of GGN by promoting unconventional termination (Stop) after addition of glycine and then allowing continued translation (Go)

Author(s) -

John F. Atkins,

Norma M. Wills,

Gary Loughran,

Chih-yu Wu,

Krishna Parsawar,

Martin D. Ryan,

Chung-Hsiung Wang,

Chad Nelson

Publication year - 2007

Publication title -

rna

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.037

H-Index - 171

eISSN - 1469-9001

pISSN - 1355-8382

DOI - 10.1261/rna.487907

Subject(s) - biology , start codon , codon usage bias , genetics , stop codon , amino acid , translation (biology) , glycine , eukaryotic translation , gene , messenger rna , genome

When a eukaryotic mRNA sequence specifying an amino acid motif known as 2A is directly followed by a proline codon, two nonoverlapping proteins are synthesized. From earlier work, the second protein is known to start with this proline codon and is not created by proteolysis. Here we identify the C-terminal amino acid of an upstream 2A-encoded product from Perina nuda picorna-like virus that is glycine specified by the last codon of the 2A-encoding sequence. This is an example of recoding where 2A promotes unconventional termination after decoding of the glycine codon and continued translation beginning with the 3′ adjacent proline codon.

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