Gene silencing in Caenorhabditis elegans by transitive RNA interference | Zendy

Matthew N. Alder | Zendy; Shale Dames | Zendy; Jeffrey P. C. Gaudet | Zendy; Susan E. Mango | Zendy

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Gene silencing in Caenorhabditis elegans by transitive RNA interference

Author(s) -

Matthew N. Alder,

Shale Dames,

Jeffrey P. C. Gaudet,

Susan E. Mango

Publication year - 2003

Publication title -

rna

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.037

H-Index - 171

eISSN - 1469-9001

pISSN - 1355-8382

DOI - 10.1261/rna.2650903

Subject(s) - rna interference , biology , rna silencing , caenorhabditis elegans , gene silencing , gene , rna , genetics , rna induced silencing complex , dna directed rna interference , trans acting sirna , microbiology and biotechnology

When a cell is exposed to double-stranded RNA (dsRNA), mRNA from the homologous gene is selectively degraded by a process called RNA interference (RNAi). Here, we provide evidence that dsRNA is amplified in Caenorhabditis elegans to ensure a robust RNAi response. Our data suggest a model in which mRNA targeted by RNAi functions as a template for 5' to 3' synthesis of new dsRNA (termed transitive RNAi). Strikingly, the effect is nonautonomous: dsRNA targeted to a gene expressed in one cell type can lead to transitive RNAi-mediated silencing of a second gene expressed in a distinct cell type. These data suggest dsRNA synthesized in vivo can mediate systemic RNAi.

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