Regulated expression of functional external guide sequences in mammalian cells using a U6 RNA polymerase III promoter | Zendy

Elizaveta A. Kovrigina | Zendy; Li Yang | Zendy; Emmanuel Pfund | Zendy; Sidney Altman | Zendy

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Regulated expression of functional external guide sequences in mammalian cells using a U6 RNA polymerase III promoter

Author(s) -

Elizaveta A. Kovrigina,

Li Yang,

Emmanuel Pfund,

Sidney Altman

Publication year - 2005

Publication title -

rna

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.037

H-Index - 171

eISSN - 1469-9001

pISSN - 1355-8382

DOI - 10.1261/rna.2140505

Subject(s) - biology , microbiology and biotechnology , messenger rna , transcription (linguistics) , rna polymerase ii , rnase p , promoter , gene expression , gene , rna , genetics , philosophy , linguistics

A regulatable promoter has been stably integrated into a human embryonic kidney cell line. The promoter is a pol III mouse promoter and is under the control of ponasterone A, an ecdysone inducer. The promoter controls transcription of an external guide sequence (EGS) targeted against Rpp38, a protein subunit of human RNase P, or of lamin A/C, a gene product located in the nucleus. The amounts of protein of both gene products are severely reduced when the EGSs are made. Several other, but not all, of the protein subunits of RNase P are also inhibited in both mRNA and protein levels when Rpp38 mRNA is targeted.

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