Proteomic and functional analysis of the noncanonical poly(A) polymerase Cid14 | Zendy

Claudia Isabelle Keller Valsecchi | Zendy; Katrina Woolcock | Zendy; Daniel Heß | Zendy; Marc Bühler | Zendy

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Proteomic and functional analysis of the noncanonical poly(A) polymerase Cid14

Author(s) -

Claudia Isabelle Keller Valsecchi,

Katrina Woolcock,

Daniel Heß,

Marc Bühler

Publication year - 2010

Publication title -

rna

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.037

H-Index - 171

eISSN - 1469-9001

pISSN - 1355-8382

DOI - 10.1261/rna.2053710

Subject(s) - biology , heterochromatin , computational biology , genetics , chromatin , ribosomal protein , rna polymerase ii , zinc finger , gene , microbiology and biotechnology , ribosome , gene expression , rna , transcription factor , promoter

The fission yeast Cid14 protein belongs to a family of noncanonical poly(A) polymerases which have been implicated in a broad range of biological functions. Here we describe an extensive Cid14 protein–protein interaction network and its biochemical dissection. Cid14 most stably interacts with the zinc-knuckle protein Air1 to form the Cid14–Air1 complex (CAC). Providing a link to ribosomal RNA processing, Cid14 sediments with 60S ribosomal subunits and copurifies with 60S assembly factors. In contrast, no physical link to chromatin has been identified, although gene expression profiling revealed that efficient silencing of a few heterochromatic genes depends on Cid14 and/or Air1.

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