Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs | Zendy

Christian Cole | Zendy; Andrew Sobala | Zendy; Cheng Lü | Zendy; Shawn Thatcher | Zendy; Andrew Bowman | Zendy; John W. Brown | Zendy; Pamela J. Green | Zendy; Geoffrey J. Barton | Zendy; György Hutvàgner | Zendy

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Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs

Author(s) -

Christian Cole,

Andrew Sobala,

Cheng Lü,

Shawn Thatcher,

Andrew Bowman,

John W. Brown,

Pamela J. Green,

Geoffrey J. Barton,

György Hutvàgner

Publication year - 2009

Publication title -

rna

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.037

H-Index - 171

eISSN - 1469-9001

pISSN - 1355-8382

DOI - 10.1261/rna.1738409

Subject(s) - biology , small nucleolar rna , dicer , rna , small rna , transfer rna , small nuclear rna , deep sequencing , computational biology , non coding rna , genetics , small interfering rna , gene , genome

Deep sequencing technologies such as Illumina, SOLiD, and 454 platforms have become very powerful tools in discovering and quantifying small RNAs in diverse organisms. Sequencing small RNA fractions always identifies RNAs derived from abundant RNA species such as rRNAs, tRNAs, snRNA, and snoRNA, and they are widely considered to be random degradation products. We carried out bioinformatic analysis of deep sequenced HeLa RNA and after quality filtering, identified highly abundant small RNA fragments, derived from mature tRNAs that are likely produced by specific processing rather than from random degradation. Moreover, we showed that the processing of small RNAs derived from tRNA(Gln) is dependent on Dicer in vivo and that Dicer cleaves the tRNA in vitro.

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