Deep sequencing identifies new and regulated microRNAs in Schmidtea mediterranea | Zendy

Yi-Chien Lu | Zendy; Magda Smielewska | Zendy; Dasaradhi Palakodeti | Zendy; Michael Lovci | Zendy; Stefan Aigner | Zendy; G Yeo | Zendy; Brenton R. Graveley | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Deep sequencing identifies new and regulated microRNAs in Schmidtea mediterranea

Author(s) -

Yi-Chien Lu,

Magda Smielewska,

Dasaradhi Palakodeti,

Michael Lovci,

Stefan Aigner,

G Yeo,

Brenton R. Graveley

Publication year - 2009

Publication title -

rna

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.037

H-Index - 171

eISSN - 1469-9001

pISSN - 1355-8382

DOI - 10.1261/rna.1702009

Subject(s) - biology , planarian , microrna , regeneration (biology) , deep sequencing , stem cell , planaria , somatic cell , function (biology) , induced pluripotent stem cell , microbiology and biotechnology , lineage (genetic) , genetics , computational biology , gene , embryonic stem cell , genome

MicroRNAs (miRNAs) play important roles in directing the differentiation of cells down a variety of cell lineage pathways. The planarian Schmidtea mediterranea can regenerate all lost body tissue after amputation due to a population of pluripotent somatic stem cells called neoblasts, and is therefore an excellent model organism to study the roles of miRNAs in stem cell function. Here, we use a combination of deep sequencing and bioinformatics to discover 66 new miRNAs in S. mediterranea. We also identify 21 miRNAs that are specifically expressed in either sexual or asexual animals. Finally, we identified five miRNAs whose expression is sensitive to gamma-irradiation, suggesting they are expressed in neoblasts or early neoblast progeny. Together, these results increase the known repertoire of S. mediterranea miRNAs and identify numerous regulated miRNAs that may play important roles in regeneration, homeostasis, neoblast function, and reproduction.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research