Open AccessPeptide release on the ribosome depends critically on the 2′ OH of the peptidyl–tRNA substrate
Author(s) -
Julie L Brunelle,
Jeffrey Jon Shaw,
Elaine M. Youngman,
Rachel Green
Publication year - 2008
Publication title -
rna
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.037
H-Index - 171
eISSN - 1469-9001
pISSN - 1355-8382
DOI - 10.1261/rna.1057908
Subject(s) - peptidyl transferase , ribosome , transfer rna , biology , ribosomal rna , peptide , translation (biology) , p site , protein subunit , substrate (aquarium) , biochemistry , stereochemistry , rna , chemistry , messenger rna , gene , ecology
Peptide release on the ribosome is catalyzed by protein release factors (RFs) on recognition of stop codons positioned in the A site of the small ribosomal subunit. Here we show that the 2′ OH of the peptidyl–tRNA substrate plays an essential role in catalysis of the peptide release reaction. These observations parallel earlier studies of the mechanism of the peptidyl transfer reaction and argue that related mechanisms are at the heart of catalysis for these reactions.
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