Growth of Nosema cuniculi in established cell lines | Zendy

T. Waller | Zendy

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Growth of Nosema cuniculi in established cell lines

Author(s) -

T. Waller

Publication year - 1975

Publication title -

laboratory animals

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.62

H-Index - 63

eISSN - 1758-1117

pISSN - 0023-6772

DOI - 10.1258/002367775780994835

Subject(s) - nosema , spore , inoculation , biology , laboratory flask , kidney , cell culture , veterinary medicine , microbiology and biotechnology , virology , immunology , chemistry , medicine , microsporidia , genetics , endocrinology

Growth patterns of Nosema cuniculi ( Encephalitozoon cuniculi) in cell cultures of bovine kidney, canine kidney, feline lung, and rabbit kidney were studied. All cell cultures used were easy to manage and the last 3 are commercially-available established cell lines. The dog kidney cells were the most suitable for large-scale production of Nosema. When grown in plastic flasks with a bottom area of 75 cm 2 , the weekly yield from Nosema-infected canine kidney cells during the 10th to 17th week after inoculation was between 4·1 x 10 7 and 9·9 x 10 7 spores per flask. An equilibrium was obtained between the Nosema infection and the kidney cells during this time. A simple method for estimating the numbel of harvested spores is also described.

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