Effects of Nipradilol, a Nitric Oxide-Releasing β -Adrenoceptor Blocking Agent, on Phosphoenolpyruvate Carboxykinase Gene Transcription in a Rat Hepatoma Cell Line | Zendy

Keishi Yamauchi | Zendy; Koji Nakajima | Zendy; Sachiko Ikeo | Zendy; Yoshihiro Nishimura | Zendy; Mitsuhisa Komatsu | Zendy; Toru Aizawa | Zendy; Kiyoshi Hashizume | Zendy

Open Access

Effects of Nipradilol, a Nitric Oxide-Releasing β -Adrenoceptor Blocking Agent, on Phosphoenolpyruvate Carboxykinase Gene Transcription in a Rat Hepatoma Cell Line

Author(s) -

Keishi Yamauchi,

Koji Nakajima,

Sachiko Ikeo,

Yoshihiro Nishimura,

Mitsuhisa Komatsu,

Toru Aizawa,

Kiyoshi Hashizume

Publication year - 2001

Publication title -

the japanese journal of pharmacology

Language(s) - English

Resource type - Journals

eISSN - 1347-3506

pISSN - 0021-5198

DOI - 10.1254/jjp.87.83

Subject(s) - phosphoenolpyruvate carboxykinase , transcription (linguistics) , nitric oxide , chemistry , biology , microbiology and biotechnology , endocrinology , biochemistry , gene , philosophy , linguistics

Effects of nipradilol, a beta-adrenoceptor blocker with a nitroxy moiety, on phosphoenolpyruvate carboxykinase (PEPCK) gene transcription were examined using a rat hepatoma cell line, H4IIE cells. Dexamethasone was employed as an enhancer of PEPCK gene transcription. Nipradilol, but not timolol (a beta-blocker without a nitroxy moiety), attenuated PEPCK gene transcription both in the control and the dexamethasone-treated cells. The effects of nipradilol were eradicated by methylene blue (an inhibitor of cellular guanylate cyclase). Nipradilol is a unique beta-blocker that suppresses PEPCK gene transcription in hepatocytes likely through liberation of nitric oxide and resultant activation of guanylate cyclase.

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