Protective Effects of Puerariae flos Against Ethanol-Induced Apoptosis on Human Neuroblastoma Cell Line SK-N-MC | Zendy

Mi-Hyeon Jang | Zendy; Min-Chul Shin | Zendy; YounJung Kim | Zendy; Joo-Ho Chung | Zendy; SungVin Yim | Zendy; Ee-Hwa Kim | Zendy; Yeunhee Kim | Zendy; ChangJu Kim | Zendy

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Protective Effects of Puerariae flos Against Ethanol-Induced Apoptosis on Human Neuroblastoma Cell Line SK-N-MC

Author(s) -

Mi-Hyeon Jang,

Min-Chul Shin,

YounJung Kim,

Joo-Ho Chung,

SungVin Yim,

Ee-Hwa Kim,

Yeunhee Kim,

ChangJu Kim

Publication year - 2001

Publication title -

the japanese journal of pharmacology

Language(s) - English

Resource type - Journals

eISSN - 1347-3506

pISSN - 0021-5198

DOI - 10.1254/jjp.87.338

Subject(s) - apoptosis , dna fragmentation , neuroblastoma , cytotoxicity , ethanol , cell culture , chemistry , flos , flow cytometry , microbiology and biotechnology , pharmacology , biology , programmed cell death , biochemistry , in vitro , antioxidant , genetics , rutin

Puerariaeflos (PF) is an oriental medical herb for alcohol abuse. To investigate whether PF possesses protective effects against ethanol (EtOH)-induced cytotoxicity in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric analysis, DNA fragmentation assay, and reverse transcription-polymerase chain reaction were performed on SK-N-MC human neuroblastoma cells. Cells treated with EtOH exhibited several apoptotic features, while those pre-treated with PF prior to EtOH exposure showed a decreased occurrence of apoptotic features. In addition, PF pre-treatment inhibited the EtOH-induced increase in caspase-3 mRNA expression. These results suggest that PF may exert protective effects against EtOH-induced apoptosis in human neuroblastoma cells.

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