Open AccessProtective Effects of Puerariae flos Against Ethanol-Induced Apoptosis on Human Neuroblastoma Cell Line SK-N-MC
Author(s) -
Mi Hyeon Jang,
Min Chul Shin,
Youn Jung Kim,
Joo Ho Chung,
Sung Vin Yim,
Ee Hwa Kim,
Yeunhee Kim,
Sang Hoon Kim
Publication year - 2001
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.87.338
Subject(s) - apoptosis , dna fragmentation , neuroblastoma , cytotoxicity , ethanol , cell culture , chemistry , flos , flow cytometry , microbiology and biotechnology , pharmacology , biology , programmed cell death , biochemistry , in vitro , antioxidant , genetics , rutin
Puerariaeflos (PF) is an oriental medical herb for alcohol abuse. To investigate whether PF possesses protective effects against ethanol (EtOH)-induced cytotoxicity in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric analysis, DNA fragmentation assay, and reverse transcription-polymerase chain reaction were performed on SK-N-MC human neuroblastoma cells. Cells treated with EtOH exhibited several apoptotic features, while those pre-treated with PF prior to EtOH exposure showed a decreased occurrence of apoptotic features. In addition, PF pre-treatment inhibited the EtOH-induced increase in caspase-3 mRNA expression. These results suggest that PF may exert protective effects against EtOH-induced apoptosis in human neuroblastoma cells.