Open AccessEffects of β-Casomorphins on Neuronal Survival in Culture of Embryonic Chick Dorsal Root Ganglion Neurons
Author(s) -
Minoru Sakaguchi,
Takeshi Fujimori,
Tomoko Satoh,
Eiko Matsumura
Publication year - 2001
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.86.363
Subject(s) - dorsal root ganglion , beta (programming language) , (+) naloxone , beta endorphin , embryonic stem cell , receptor , pertussis toxin , biology , medicine , endocrinology , opioid , microbiology and biotechnology , chemistry , pharmacology , dorsum , anatomy , g protein , biochemistry , gene , computer science , programming language
We studied the effects of beta-casomorphins (beta-CMs, mu-acting opioid peptides from milk protein beta-casein) on survival of primary-cultured chick dorsal root ganglion neurons in the presence of nerve growth factor. Beta-CM-5 and beta-CM-7 had potent neuronal survival-promoting activities. Beta-CM-4 amide (morphiceptin) and des-Tyr'-beta-CM-7 also exhibited the similar promoting effects, although their effects were very weak. The promoting effect of beta-CM-5 was prevented by co-administration of naloxone, or pretreatment with pertussis toxin. These results suggest that the neuronal survival-promoting effects of beta-CMs might be mediated through opioid receptors coupled to G proteins.