Open AccessGTPγS-Induced Ca2+ Activated Cl− Currents: Its Stable Induction by Gqα Overexpression in Xenopus Oocytes
Author(s) -
Muneshige Kaibara,
Yoshihisa Nagase,
Osamu Murasaki,
Yasuhito Uezono,
Yoshiyuki Doi,
Kohtaro Taniyama
Publication year - 2001
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.86.244
Subject(s) - gtpgammas , xenopus , guanosine , gtp' , chemistry , alpha (finance) , microbiology and biotechnology , medicine , endocrinology , g protein , biology , biochemistry , signal transduction , gene , enzyme , construct validity , nursing , patient satisfaction
In native Xenopus oocytes, injection of guanosine 5'-O-(3-thiotriphosphate) (GTPgammaS) (30 mM, 5 nl) did not induce Cl- current in 11 out of 22 oocytes. Injection of increased concentration of GTPgammaS (100 mM, 5 nl) into the oocytes induced Cl- currents in 16 out of 17 oocytes; however, the size of the induced currents was extremely varied. In oocytes overexpressing Gq alpha, GTPgammaS (30 mM, 5 nl) faithfully evoked Ca2+-activated Cl- currents. These results indicate that heterogeneous expression of Gq alpha in Xenopus oocytes provides a useful system for studying the functional roles of Gq alpha in regulating cellular events.