Open AccessSerotonin Induces Apoptosis in PGT-β Pineal Gland Tumor Cells
Author(s) -
Sung-Vin Yim,
Kyung-Hwan Kim,
ChangJu Kim,
JooHo Chung
Publication year - 2000
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - Uncategorized
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.84.71
Subject(s) - serotonin , tunel assay , apoptosis , dapi , programmed cell death , terminal deoxynucleotidyl transferase , in situ nick end labeling , biology , pineal gland , microbiology and biotechnology , endocrinology , medicine , melatonin , biochemistry , receptor
Serotonin-induced neuronal cell death has been implicated as a possible cause of neurodegenerative and neuropsychiatric disorders. To investigate the involvement of serotonin-induced apoptosis as a potential mechanism in the pathophysiology of serotonin-related diseases, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, DAPI (4',6-diamidino-2-phenylindole) staining, TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling) assay and flow cytometric analysis were performed using the immortalized pineal cell line PGT-beta. Through morphological and biochemical analyses, it was demonstrated that cell death induced by serotonin in PGT-beta cells shows classic apoptotic features. These data suggest that serotonin induces apoptosis in PGT-beta cells.