Serotonin Induces Apoptosis in PGT-β Pineal Gland Tumor Cells | Zendy

SungVin Yim | Zendy; Kyung Hwan Kim | Zendy; ChangJu Kim | Zendy; Joo-Ho Chung | Zendy

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Serotonin Induces Apoptosis in PGT-β Pineal Gland Tumor Cells

Author(s) -

SungVin Yim,

Kyung Hwan Kim,

ChangJu Kim,

Joo-Ho Chung

Publication year - 2000

Publication title -

the japanese journal of pharmacology

Language(s) - Uncategorized

Resource type - Journals

eISSN - 1347-3506

pISSN - 0021-5198

DOI - 10.1254/jjp.84.71

Subject(s) - serotonin , tunel assay , apoptosis , dapi , programmed cell death , terminal deoxynucleotidyl transferase , in situ nick end labeling , biology , pineal gland , microbiology and biotechnology , endocrinology , medicine , melatonin , biochemistry , receptor

Serotonin-induced neuronal cell death has been implicated as a possible cause of neurodegenerative and neuropsychiatric disorders. To investigate the involvement of serotonin-induced apoptosis as a potential mechanism in the pathophysiology of serotonin-related diseases, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, DAPI (4',6-diamidino-2-phenylindole) staining, TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling) assay and flow cytometric analysis were performed using the immortalized pineal cell line PGT-beta. Through morphological and biochemical analyses, it was demonstrated that cell death induced by serotonin in PGT-beta cells shows classic apoptotic features. These data suggest that serotonin induces apoptosis in PGT-beta cells.

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