Open AccessCa2+-Sensitization of Contraction in the h1 Calponin-Deficient Smooth Muscle
Author(s) -
Ryo Yoshimoto,
Masaru Hori,
Katsuhito Takahashi,
Shun′ichiro Taniguchi,
Motoya Katsuki,
Hiroshi Ozaki,
Hideaki Karaki
Publication year - 2000
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.84.474
Subject(s) - calponin , contraction (grammar) , sensitization , smooth muscle , muscle contraction , chemistry , biophysics , microbiology and biotechnology , medicine , endocrinology , neuroscience , biology
Role of h1 calponin on Ca2+-sensitivity of smooth muscle contraction was investigated using h1 calponin gene-deficient mice (CP -/-) and wild type mice (CP +/+). PGF2. induced a comparable force in intact aorta of CP +/+ and CP -/-. DPB showed similar effects to PGF2alpha. In membrane-permeabilized ileal smooth muscle, PDBu enhanced Ca2+-sensitivity of contraction comparably in CP +/+ and CP -/-. GTPgamma-S showed similar effects. Our results suggest that h1 calponin does not regulate Ca2+-sensitivity in the contractile mechanism of smooth muscle.