Open AccessThe Effects of Melatonin in Glutamate-Induced Neurotoxicity of Rat Cerebellar Granular Cell Culture
Author(s) -
Akçahan Gepdíremen,
Selma Düzenli,
Ahmet Hacımüftüoğlu,
Dilek Bulucu,
Halis Süleyman
Publication year - 2000
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.84.467
Subject(s) - neurotoxicity , melatonin , glutamate receptor , neuroprotection , pharmacology , glutamic acid , chemistry , cerebellum , programmed cell death , biology , medicine , endocrinology , toxicity , biochemistry , apoptosis , amino acid , receptor
In the present study, melatonin was tested in subsequent doses in glutamate induced neurotoxicity in cerebellar granular cell culture of rat pups. Glutamate at 10(-7) M was found to induce neuronal cell death. The dead cell score was 2.75+/-0.7 in the control, while it was found to be 35.12+/-1.8 in the glutamate-administered group (P<0.0001). Melatonin very potently blocked glutamate neurotoxicity at all doses tested, with 10(-3) M, the highest dose tested, being the most effective. Glutamate may exert a neuroprotective effect by blocking one or more steps of the oxidation cascade in neurons and this effect may be blocked by melatonin.