Open AccessEffect of APGW-Amide on [Ca2+]i in Rat Pheochromocytoma PC12 Cells
Author(s) -
Xiao Han,
Yuzuru Ito,
Yoshinori Nozawa,
Yi-Fan Jiang,
Kazutoshi Matsunami
Publication year - 2000
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.84.355
Subject(s) - egta , amide , extracellular , protein kinase c , chemistry , channel blocker , medicine , endocrinology , biophysics , microbiology and biotechnology , calcium , biochemistry , biology , kinase , organic chemistry
In order to determine whether Ala-Pro-Gly-Try-NH2 (APGW-amide) could affect mammalian excitable cells, we investigated the effect of APGW-amide in PC12 cells. APGW-amide caused a rapid [Ca2+]i elevation, which was completely prevented by elimination of extracellular Ca2+ with EGTA and inhibited by two L-type Ca2+ channel blockers. [Ca2+]i elevation was also blocked by a specific PKC inhibitor and prolonged pretreatment of cells with PMA. These results indicate that APGW-amide elevates [Ca2+]i in PC12 cells, possibly by Ca2+ influx via L-type Ca2+ channel activated by PKC.