Effect of APGW-Amide on [Ca2+]i in Rat Pheochromocytoma PC12 Cells | Zendy

Xiao Han | Zendy; Yuzuru Ito | Zendy; Yoshinori Nozawa | Zendy; Yifa Jiang | Zendy; Ken’ichi Matsunami | Zendy

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Effect of APGW-Amide on [Ca2+]i in Rat Pheochromocytoma PC12 Cells

Author(s) -

Xiao Han,

Yuzuru Ito,

Yoshinori Nozawa,

Yifa Jiang,

Ken’ichi Matsunami

Publication year - 2000

Publication title -

the japanese journal of pharmacology

Language(s) - English

Resource type - Journals

eISSN - 1347-3506

pISSN - 0021-5198

DOI - 10.1254/jjp.84.355

Subject(s) - egta , amide , extracellular , protein kinase c , chemistry , channel blocker , medicine , endocrinology , biophysics , microbiology and biotechnology , calcium , biochemistry , biology , kinase , organic chemistry

In order to determine whether Ala-Pro-Gly-Try-NH2 (APGW-amide) could affect mammalian excitable cells, we investigated the effect of APGW-amide in PC12 cells. APGW-amide caused a rapid [Ca2+]i elevation, which was completely prevented by elimination of extracellular Ca2+ with EGTA and inhibited by two L-type Ca2+ channel blockers. [Ca2+]i elevation was also blocked by a specific PKC inhibitor and prolonged pretreatment of cells with PMA. These results indicate that APGW-amide elevates [Ca2+]i in PC12 cells, possibly by Ca2+ influx via L-type Ca2+ channel activated by PKC.

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