Open AccessEffects of Zaldaride Maleate, an Antidiarrheal Compound, on Intracellular Ca2+-Mediated Short-Circuit Current Response in the Rat Colonic Mucosa.
Author(s) -
Nobuo Aikawa,
Akira Karasawa,
Kenji Ohmori
Publication year - 2000
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.83.86
Subject(s) - tetrodotoxin , acetylcholine , intracellular , chemistry , stimulation , medicine , endocrinology , pharmacology , biology , biochemistry
The effect of zaldaride maleate (ZAL) on intracellular Ca2+-mediated short-circuit current (Isc) response in rat colonic mucosa was investigated. The Isc response to acetylcholine was partially reduced by the neuronal blocker tetrodotoxin. The tetrodotoxin-sensitive Isc response to acetylcholine was inhibited by ZAL. The tetrodotoxin-insensitive effect, which indicates direct stimulation of acetylcholine in colonic mucosa, was not affected by ZAL. The Isc response to the Ca2+ ionophore A23187 was reduced by ZAL in the presence of tetrodotoxin. These results suggest that ZAL reduces colonic ion secretion by inhibiting a tetrodotoxin-sensitive neuronal pathway and an intracellular Ca2+ pathway in epithelium.