Serum-Deprivation Induces Cell Death of Rat Cultured Microglia Accompanied With Expression of Bax Protein. | Zendy

Yutaka Kōyama | Zendy; Yuji Kimura | Zendy; Yasuhiro Yoshioka | Zendy; Daisuke Wakamatsu | Zendy; Ryohei Kozaki | Zendy; Hitoshi Hashimoto | Zendy; Toshio Matsuda | Zendy; Akemichi Baba | Zendy

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Serum-Deprivation Induces Cell Death of Rat Cultured Microglia Accompanied With Expression of Bax Protein.

Author(s) -

Yutaka Kōyama,

Yuji Kimura,

Yasuhiro Yoshioka,

Daisuke Wakamatsu,

Ryohei Kozaki,

Hitoshi Hashimoto,

Toshio Matsuda,

Akemichi Baba

Publication year - 2000

Publication title -

the japanese journal of pharmacology

Language(s) - English

Resource type - Journals

eISSN - 1347-3506

pISSN - 0021-5198

DOI - 10.1254/jjp.83.351

Subject(s) - microglia , apoptosis , programmed cell death , bax protein , cell , microbiology and biotechnology , biology , immunohistochemistry , cell culture , protein expression , immunology , gene , inflammation , biochemistry , genetics

Expression of Bcl-2 related proteins in rat microglial primary culture was examined. At relative low cell densities, serum deprivation caused cell death and nuclei condensation of cultured microglia. Expression of a pro-apoptotic protein, Bax, but not Bcl-2, was increased by the serum deprivation. SB203580, a specific inhibitor of p38MAPK, prevented both the serum deprivation-induced Bax expression and microglial death. Immunochemical staining showed that microglia expressing a high level of Bax were subjected to apoptosis-like cell death. These observations suggest that Bax expression underlies the apoptosis of cultured microglia after serum deprivation.

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