Open AccessAssessment of Genotoxicity of Dental Antiseptics. Ability of Phenol, Guaiacol, p-Phenolsulfonic Acid, Sodium Hypochlorite, p-Chlorophenol, m-Cresol or Formaldehyde to Induce Unscheduled DNA Synthesis in Cultured Syrian Hamster Embryo Cells.
Author(s) -
Fumiaki Hamaguchi,
Takeki Tsutsui
Publication year - 2000
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.83.273
Subject(s) - guaiacol , genotoxicity , sodium hypochlorite , chemistry , phenol , comet assay , hamster , formaldehyde , biochemistry , dna , hypochlorite , dna damage , pharmacology , microbiology and biotechnology , biology , toxicity , organic chemistry , enzyme
To assess the genotoxicity of seven dental antiseptics, the ability of these agents to induce unscheduled DNA synthesis (UDS) was examined using Syrian hamster embryo (SHE) cells. Treatment of SHE cells with phenol or formaldehyde induced UDS in a concentration-dependent manner as determined by direct scintillation counting of [3H]thymidine incorporated into DNA during repair synthesis. Guaiacol or m-cresol induced UDS only in the presence of exogenous metabolic activation. p-Phenolsulfonic acid, sodium hypochlorite and p-chlorophenol failed to induce UDS in the presence or absence of exogenous metabolic activation. Our results suggest that phenol, guaiacol, m-cresol and formaldehyde are genotoxic to mammalian cells.