Open Access2-Bromoethylamine, a Suicide Inhibitor of Tissue-Bound Semicarbazide-Sensitive Amine Oxidase.
Author(s) -
Hiroyasu Kinemuchi,
Masato Jinbo,
Atsushi Tabata,
Yoshie Toyoshima,
Yasuhiko Arai,
Takeshi Tadano,
Kensuke Kisara,
Lars Oreland
Publication year - 2000
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.83.164
Subject(s) - amine oxidase , semicarbazide , amine oxidase (copper containing) , chemistry , enzyme , amine gas treating , adduct , biochemistry , covalent bond , organic chemistry , diamine oxidase
Various mammalian tissues contain plasma membrane-bound amine oxidase, termed semicarbazide-sensitive amine oxidase (SSAO). In the present study, 2-bromoethylamine has been studied with regard to inhibitory properties towards tissue-bound SSAO in rat lung. Without preincubation, 2-bromoethylamine was a competitive and reversible SSAO inhibitor with a Ki value of 2.5 microM. After preincubation, it time-dependently and non-competitively inhibited SSAO activity, probably by forming the covalently-bound enzyme-inhibitor adduct. The data presented suggest that 2-bromoethylamine may act as a suicide inhibitor of SSAO.