Open AccessCharacterization of Protease-Activated Receptors in Rat Peritoneal Mast Cells
Author(s) -
Hiroyuki Nishikawa,
Atsufumi Kawabata,
R. Kuroda,
Motohiro Nishida,
Kenzo Kawai
Publication year - 2000
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.82.74
Subject(s) - degranulation , histamine , protease activated receptor 2 , agonist , receptor , mast cell , protease activated receptor , in vivo , chemistry , protease , microbiology and biotechnology , thrombin , biology , endocrinology , medicine , immunology , enzyme , biochemistry , enzyme linked receptor , platelet
Activation of protease-activated receptor (PAR)-1 or PAR-2 elicits inflammation most probably via mast cell degranulation in vivo. The present study aimed at characterizing PARs in rat peritoneal mast cells (PMC). Messenger RNA for PAR-1, but not for PAR-2, was detected in PMC. Thrombin, the PAR-1 agonist SFLLR-NH2 or the PAR-2 agonist SLIGRL-NH2 failed to induce histamine release from PMC. Surprisingly, the PAR-2-inactive control peptide LSIGRL-NH2 triggered histamine release from PMC. Thus, PAR-1, but not PAR-2, are expressed in PMC, whereas neither PAR-1 nor PAR-2 are considered to be involved in degranulation of PMC. LSIGRL-NH2 does not appear to be appropriate as a control peptide for PAR-2 in inflammation studies.