Open AccessCytotoxic Effect of Paraquat on Rat C6 Glioma Cells: Evidence for the Possibility of Non-oxidative Damage to the Cells
Author(s) -
Kyoji Morita,
Itsuo Tokunaga,
Shinichi Kubo
Publication year - 1999
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.79.121
Subject(s) - paraquat , cytotoxic t cell , chemistry , glioma , oxidative phosphorylation , apoptosis , viability assay , deoxyguanosine , oxidative stress , oxidative damage , cytotoxicity , pharmacology , biology , microbiology and biotechnology , biochemistry , cancer research , in vitro
Although paraquat has been shown to cause oxidative damage to neuronal cells, little is known about its effect on glial cells. Thus the effect of paraquat on glial cells was examined using rat C6 glioma cells as a model system. Paraquat reduced cell viability in a concentration- and time-dependent manner, and this toxic effect was not significantly attenuated by various kinds of antioxidants. Furthermore, paraquat failed to increase 8-hydroxy-deoxyguanosine formation in the cells. These results indicate that paraquat can be toxic to glial cells and suggest that this cytotoxic effect may not be associated with the oxidative damage to the cells.