Open AccessIn Vivo Microdialysis Assessment of Nerve-Stimulated Contractions Associated With Increased Acetylcholine Release in the Dog Intestine
Author(s) -
Akira Furuichi,
Noriaki Makimoto,
Masayuki Ogishima,
Kanichirou Nakao,
Akihito Enjoji,
Junichiro Furui,
Takashi Kanematsu,
Kohtaro Taniyama
Publication year - 1999
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.79.109
Subject(s) - acetylcholine , microdialysis , tetrodotoxin , in vivo , atropine , cholinergic , contractility , stimulation , endocrinology , medicine , chemistry , endogeny , small intestine , muscarinic acetylcholine receptor , biology , receptor , microbiology and biotechnology
Intestinal contractility and release of endogenous acetylcholine (ACh) were measured simultaneously in vivo in the small intestine of the anesthetized dog. Electrical stimulation of nerves in the intestinal seromuscular layers caused contractions and increased concentrations of ACh in the dialysate, which were abolished by infusion of tetrodotoxin into the intestinal marginal artery at 75 nmol/ml. Intraarterial administration of atropine at 150 nmol/ml abolished the stimulated contractions, without significant effects on increases in concentrations of dialysate ACh. Thus, the nerve-stimulated contractions were found in vivo to be associated with a local increase in ACh release from the intestinal cholinergic neurons.