Open AccessProduction of Superoxide and Nitric Oxide by Alveolar Macrophages in the Bleomycin-Induced Interstitial Pneumonia Mice Model
Author(s) -
C. Yamazaki,
Jiro Hoshino,
Tomoko Sekiguchi,
Yusuke Hori,
Satoshi Miyauchi,
Syoji Mizuno,
Katsuyuki Horie
Publication year - 1998
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.78.69
Subject(s) - peroxynitrite , superoxide , bleomycin , nitric oxide , nitrotyrosine , pulmonary fibrosis , pathogenesis , pathology , lung , immunostaining , chemistry , fibrosis , hydroxyproline , nitric oxide synthase , medicine , immunohistochemistry , biochemistry , chemotherapy , organic chemistry , enzyme
To elucidate the potential role of superoxide (O2-) and nitric oxide (NO) in the pathogenesis of interstitial pneumonia, the quantity of O2- and NO produced by the alveolar macrophages (AM) were determined in the bleomycin (BLM)-induced interstitial pneumonia mouse model. The production of O2- and NO increased on days 7, 14 and 21 after BLM injection. Strong expression of peroxynitrite (ONOO-) was seen in AM by using immunostaining for nitrotyrosine. The hydroxyproline contents increased on day 21 after BLM injection. O2- and NO are thought to play an important role in the pathology of fibrosis.