Open AccessA Single Stranded DNA-Binding Protein, ssCRE-BP/Purα, in Rat Lung and Its Increase in Allergic Airway Inflammation
Author(s) -
Er-Qing Wei,
Yoshihito Irie,
CheHui Kuo,
Yun Ding,
Sanyong Niu,
Eunju Do,
Naomasa Miki
Publication year - 1998
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.78.419
Subject(s) - ovalbumin , immunohistochemistry , inflammation , in situ hybridization , lung , allergic inflammation , microbiology and biotechnology , biology , immunology , antigen , chemistry , pathology , gene expression , gene , medicine , biochemistry
ssCRE-BP/Pur alpha is a single stranded DNA-binding protein and may be involved in gene replication and transcription and in the development of morphine dependence. We found a ssCRE-BP/Pur alpha (45 kDa) in rat lung that was larger than those (40 kDa) identified in rat and mouse brains and mouse lung. Immunohistochemistry showed that ssCRE-BP/Pur alpha is primarily distributed in the lung epithelium. As allergic inflammation induces various gene expressions, we investigated the changes of Pur alpha during airway inflammation. Ovalbumin-sensitized rats were used for inducing allergic airway inflammation. The expression and DNA-binding activity of 45-kDa ssCRE-BP/Pur alpha were significantly increased in the sensitized rat lungs 24 hr after antigen challenge, but not in those of rats nonsensitized or sensitized with ovalbumin and challenged with saline. Immunohistochemistry and in situ hybridization demonstrated that the vascular endothelial cells and numerous infiltrated eosinophils around the airways were stained with anti-Pur alpha antibody. These data suggest that rat lung and the eosinophils contain a 45-kDa ssCRE-BP/Pur alpha that is increased when airway inflammation occurs.