Open AccessEffect of Gadolinium Chloride Treatment on Concanavalin A-Induced Cytokine mRNA Expression in Mouse Liver
Author(s) -
Toshihiro Okamoto,
Osamu Maeda,
Naoki Tsuzuike,
K. Hara
Publication year - 1998
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.78.101
Subject(s) - concanavalin a , kupffer cell , cytokine , tumor necrosis factor alpha , chemistry , endocrinology , medicine , microbiology and biotechnology , biology , biochemistry , in vitro
The effect of Kupffer cell depression on concanavalin A (Con A)-induced cytokine mRNA expression in the liver was studied. Gadolinium chloride (GdCl3) is a commonly used Kupffer cell inhibitor. GdCl3 (40 mg/kg, i.p.) was injected into each mouse, and 24 hr later, Con A (0.2 mg/mouse) was administered. Plasma was obtained at 24 hr after Con A treatment for alanine aminotransferase (ALT) measurement. GdCl3 treatment inhibited Con A-induced elevation of ALT. However, it did not inhibit Con A-induced interleukin-2 or tumor necrosis factor-alpha mRNA expression. The present results suggest that Kupffer cells are not responsible for Con A-induced cytokine expression in the liver.