Open AccessEffect of Growth Factor on GTPase-Activating Protein (Ras GAP) in Chinese Hamster Ovary Cells
Author(s) -
Hidenori Sasa,
Hiroko Nakata,
Tadashi Umekage,
Misako Namima,
Koji Tomiyama,
Shinya Arimura,
Mitsunao Kobayashi,
Yasuhiro Watanabe
Publication year - 1998
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.76.121
Subject(s) - chinese hamster ovary cell , gtpase activating protein , gtpase , microbiology and biotechnology , biology , ovary , signal transduction , cell culture , gtp' , endocrinology , g protein , biochemistry , enzyme , genetics
GTPase-activating proteins (GAPs) stimulate the hydrolysis of GTP bound to small G-proteins and regulate the signal transduction pathway. Changes in the expression of p21-Ras p120-GAP induced by growth factor treatment were examined in cultured Chinese hamster ovary (CHO) and human choriocarcinoma (BeWo) cells. Expression of p120-GAP and GAP activity were measured. Fetal bovine serum induced a significant increased level of GAP in CHO cells, but did not increase GAP in BeWo cells. The results suggest that growth factors affect Ras GAP expression in CHO cells, while they do not in other cells such as BeWo cells.