Open AccessPlatelet-Derived Growth Factor Blocks the Cell-Cycle Transition from the G0 to G1 Phase in Subcultured Angiogenic Endothelial Cells in Rat Thoracic Aorta.
Author(s) -
Ikuko Kimura,
Hiroshi Tsuneki,
Motonori Okabe,
M. Ogasawara
Publication year - 1997
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.74.303
Subject(s) - platelet derived growth factor receptor , platelet derived growth factor , cell cycle , growth factor , microbiology and biotechnology , biology , cell growth , flow cytometry , angiogenesis , medicine , endocrinology , cell , chemistry , receptor , cancer research , biochemistry
Platelet-derived growth factor (PDGF)-BB induces tube formation by the differentiating (tube-forming) endothelial cells (EC) of rat thoracic aorta, although PDGF-BB does not affect the proliferative EC (increasing the cell numbers) at the progression phase. These changes in the responses to PDGF-BB were due to the phenotype-dependent expression of PDGF beta-receptor (PDGFR-beta) on EC because PDGFR-beta-like immunoreactivity was observed in the angiogenic EC forming a tube-like structure in 35-day culture with 10% fetal bovine serum, but not in the proliferative EC in 5-day culture. To elucidate the functional role of PDGFR-beta in the alteration of EC phenotype, the influence of PDGF-BB on the cell cycle of EC was investigated by flow cytometry. This analysis demonstrates that PDGF-BB blocks the transition from the G0 to G1 phase in the 35-day cultured EC, although no effect was observed on any phases of the cell cycle in 5-day culture. We conclude that 1) PDGFR-beta is expressed in mature angiogenic EC of rat aorta, and 2) PDGF-BB may contribute to promotion of the EC differentiation with tubular morphogenesis by inhibiting cell growth.