Open AccessCa2+ Depletion Facilitates Taurine Release in Cultured Rat Astrocytes
Author(s) -
Kazuhiro Takuma,
Takehisa Matsuda,
Yugo Kishida,
Shoichi Asano,
Junichi Azuma,
Akemichi Baba
Publication year - 1996
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - Uncategorized
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.72.75
Subject(s) - taurine , chemistry , endogeny , liberation , nifedipine , tetrodotoxin , astrocyte , biophysics , calcium , biochemistry , endocrinology , microbiology and biotechnology , medicine , biology , in vitro , central nervous system , amino acid , organic chemistry
Removal of external Ca2+ facilitated endogenous taurine release in cultured rat astrocytes. The stimulated release was not affected by furosemide, sucrose, tetrodotoxin and 3,4-dichlorobenzamil, but partially inhibited by nifedipine. Omission of external Na+ increased basal taurine release, and the effects of Na+ removal and Ca2+ depletion on the release were additive. The Na(+)-free condition did not affect Ca2+ paradox-induced cell death in astrocytes. These findings suggest that Ca2+ depletion facilitates taurine release in a mechanism independent of volume and the Na+ gradient and that the release is not involved in Ca2+ paradox-induced delayed cell toxicity in astrocytes.