Open AccessOxidative Stress-Induced Increase in Intracellular Ca2+ and Ca2+-Induced Increase in Oxidative Stress: An Experimental Model Using Dissociated Rat Brain Neurons
Author(s) -
Yasuo Oyama,
Eisuke Okazaki,
Lumi Chikahisa,
Tadayoshi Nagano,
Chiharu Sadakata
Publication year - 1996
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.72.381
Subject(s) - oxidative stress , ionomycin , hydrogen peroxide , chemistry , intracellular , reactive oxygen species , oxidative phosphorylation , calcium , ionophore , biophysics , calcium in biology , biochemistry , biology , organic chemistry
In order to study the oxidative stress-induced change in intracellular concentration of Ca2+ ([Ca2+]i) and Ca(2+)-induced oxidative stress, effects of hydrogen peroxide and ionomycin, a calcium ionophore, on rat cerebellar neurons were examined using a flow cytometer and fluorescent dyes: fluo-3 for monitoring [Ca2+]i; 2',7'-dichlorofluorescin, for reactive oxygen species; and 5-chloromethylfluorescein, for cellular nonprotein thiols. Oxidative stress induced by hydrogen peroxide dose-dependently increased [Ca2+]i and decreased the content of nonprotein thiols. Ionomycin increased oxidative metabolism and decreased the content of nonprotein thiols. Results suggest that oxidative stress induces an increase in [Ca2+]i while an increase in [Ca2+]i increases oxidative stress in neurons.