Open AccessProstanoid Receptor-Mediated Calcium Signaling in Cultured Rat Astrocytes
Author(s) -
Junichi Kitanaka,
Kazuhiro Takuma,
Kaoru Kondo,
Akemichi Baba
Publication year - 1996
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - Uncategorized
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.71.85
Subject(s) - prostanoid , thromboxane a2 , stimulation , receptor , intracellular , calcium in biology , prostaglandin , astrocyte , chemistry , mobilization , calcium , fura 2 , endocrinology , medicine , biology , microbiology and biotechnology , biochemistry , central nervous system , enzyme , cytosol , history , archaeology
We investigated prostanoid-induced intracellular Ca2+ mobilization in Ca(2+)-sensitive dye fura-2-loaded cultured astrocytes. The thromboxane (TX) A2 analog STA2 (9,11-epithio-11,12-methano-TXA2) and/or prostaglandin (PG) F2 alpha (each used at 1 microM) stimulated intracellular Ca2+ mobilization in single cultured rat type 1 astrocytes. Three response patterns were observed: only STA2-sensitive, only PGF2 alpha-sensitive, and both prostanoids-sensitive cells. The Ca2+ response was prostanoid-dose-dependent (0.1 - 1 microM) and showed a rapid spike-like Ca2+ rise that peaked within 30 sec after the stimulation by the ligand. These observations suggest that type 1 astrocytes are heterogeneous with respect to the expression of receptors for TXA2 and PGF2 alpha, which are linked to Ca2+ mobilization.